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Sections were permeabilized using PBS-Triton, and blocking was carried out using 5% blocking buffer: 5% BSA, 1% goat serum (Jackson Immuno Research), 1% donkey serum (Jackson Immuno Research) in PBS-T
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The Lancet , 365 (9477), 21252136

Under LC-MS/MS conditions, there was no interfering peak during the elution of the analytes or IS in the blank matrices

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