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doi: 10.1016/j.pharep.2014.09.004

It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when theinhibition ratiois 10%~50% (theoptimalinhibition ratio is the range of 25%~45%.) before formal experiment

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Nesbitt JC, Jr PJ, Walsh GL, Ryan MB, Roth JA

In addition, there are a variety of ion channels, receptors and carrier-mediated transport (CMT) on the BBB endothelium and pericytes that mediate substance transport and information transmission, and there is close information crosstalk between the endothelium, pericytes and astrocytes (Zhao et al., 2015