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Cells were washed three times in FACS wash buffer with centrifugation

AL-C: Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Software, Supervision, Validation, Visualization, Writing original draft, Writing review & editing

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Additionally, they can suffer from a phenomenon known as "memory effect" if not discharged properly, which can reduce their lifespan