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Western blot analysis involved preparing whole cell lysates using RIPA lysis buffer, quantifying protein concentration via BCA assay, followed by SDS-PAGE, transfer to nitrocellulose membranes, incubation with primary and HRP-conjugated secondary antibodies, and detection using an ECL solution [1]

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J Biol Chem (2016) 291(16):848699

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Obesity A condition characterized by the excessive accumulation of fat in the body

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"It's possible that some 'ammonia cirrus' has formed on top of the SEB, hiding the SEB from view," Orton explained

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