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European Orlistat Obesity Study Group
To assess the gene knockdown efficiency in the context of single versus double RNAi, we performed reverse transcription, followed by real-time quantitative PCR (RTqPCR) using primers to each gene of interest (Extended Data Fig

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Under acidic conditions, sulfanilic acid reacts with nitrite to form a diazonium salt, which readily couples with N-(1-naphthyl)ethylenediamine, creating a highly colored azo dye detectable at 548 nm (Fig

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Chinese Journal of Cardiology