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First, the distal heme His64 (HisE7) must unbind from the heme iron to allow ligand binding (FIGURE 1)

Cells were treated either with DMSO or various concentrations of RSL3 (0.53.0 M) for 48 h

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At pH 5.5, GLP-1 WT showed slightly weaker binding to FcRn than Tanzeum, whereas GLP-1 LX demonstrated reduced receptor binding (Fig

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