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UbiCRest Deubiquitylation reactions were performed in reaction buffer (50 mM Tris-HCl pH 7.5, 100 mM NaCl, 10 mM MgCl 2 , 2 mM -mercaptoethanol) contained 25100 nM AMSH*, 50200 nM OTUB1* 100020,000 ng Ribosome, 100 ng K48-Ub4 (UBPBio), 100 ng K63-Ub4 (UBPBio)

Um, and I know that in my dad's practice, he also had, you know, he was a first mover on a lot of things, and he used to tell us at home, um, you know, about all the cool stuff he saw at the a a o or wherever he (00:18:00) was going, and, you know, obviously wants to bring it

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By comparing these transcriptional changes with those induced by an inactive analogue, C1-14 (Supplementary Fig