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A thio-sugar auxiliary, incorporated as a glycosyl amino acid during solid-phase peptide synthesis, facilitates amide bond formation near the ligation site via thiol exchange with a peptidyl thioester

Next, 4-phosphopantothenic acid is converted to 4-phosphopantothenoylcysteine with 4-phosphopantothenic acid synthetase requiring CTP and cysteine and releasing CDP and inorganic phosphate

This hypothesis was formulated based on an experiment in which an antisense MRP2 RNA construct was introduced into human hepatocellular carcinoma HepG2 cells, resulting in enhanced sensitivity to several anticancer drugs such as cisplatin, vincristine, doxorubicin, and the camptothecin derivatives CPT-11 and SN-38[57]

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This property creates a specific storage challenge