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Expression Profiling of Spinal Genes in Peripheral Neuropathy Model Rats with Type 2 Diabetes Mellitus

2.2 Preparation of crude purified hemoglobin For purified hemoglobin preparation, pRBCs tube was placed in a dry-ice acetone bath for few seconds until the PRBCs freeze and visibly seen as solid

This is consistent with a previous report that the core apoptotic pathway genes, including CED-9/BCL-1, CED-4/APAF-1 and cell-killing caspase ced-3 , are not required for axonal fusion 16

however, phospholipase A2 can mitigate these effects (Hossen et al., 2017a)

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Additionally, ethical concerns related to stem cell sourcing and genetic modification pose ongoing risks, mandating rigorous compliance that can slow clinical trial progression by 6-12 months on average, impacting market timelines