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Importantly, this design enabled the production of a library of stapled TF analogs derived from the DNA-binding domain (DBD) of Max with selective incorporation of staples at strategic positions in the leucine zipper and the basic domain

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[DOI] [PMC free article] [PubMed] [Google Scholar] 182.Back P., Braeckman B.P., Matthijssens F

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During pre-clearing step, the cell-lysates are pre-cleared of proteins that bind to antibodies non-specifically using an isotype control antibody

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10.1186/s12943-019-1089-9

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