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Methods Mol Biol 112:531552

This molecule was duplexed and amplified by 5 cycles of PCR using Phusion HF polymerase (New England Biolabs, NEB), then inserted into blunt II TOPO vector (Invitrogen) to generate a plasmid for electroporation efficiency control (pSSBR)

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By contrast, the labile zinc pool, associated with low-molecular-weight ligands and metallothioneins (MTs), supports signaling and transfer reactions 29

doi: 10.3390/antiox10121859