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5-LOX in Alzheimers Disease: Potential Serum Marker and In Vitro Evidences for Rescue of Neurotoxicity by Its Inhibitor YWCS
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Kim and Kim, 2005)

The degeneration of neurons in the ventral tegmental area, accompanied by reduced dopamine release and impaired connectivity in target regions, is recognized as a key feature of the early stages of AD, preceding even the formation of A plaques
After three rinses in PBS (10 min each), the tissues were incubated with secondary antibodies in PBS containing 5% BSA at room temperature for 2 h, washed three times with PBS, mounted using Mowiol mounting medium, and imaged under a fluorescence microscope

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