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Then, the slides were incubated with mouse primary Nrf2 antibody (Proteintech, 66504-1-lg) and rabbit primary PKM2 antibody (Cell Signaling Technology, 4053) at 4 C overnight and then with PLA probe solution for 1 h at 37 C

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Novel insights into cuproptosis in alcoholic liver disease using bioinformatics analysis and experimental validation