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Crude peptides were first purified by reverse phase HPLC using a CI 8 column using gradient elution

This infection leads to inflammatory demyelination in brain and spinal cord, with a mixture of cluster of differentiation (CD)4 + and CD8 + T-cells, B-cells, microglia, and macrophages contributing to the demyelination [14]

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Initial studies on NIH-3T3 fibroblasts and U937 histiocytic lymphoma cells show that GHK-Cu and GHK-PEG stimulate fibroblast proliferation while attenuating U937 cell proliferation

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