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Once the bacteria had been heat-shocked, they we plated on plates containing kanamycin, IPTG and x-gal

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however, the ontogeny, molecular determinants, and functional consequences of this heterogeneity remain incompletely understood, particularly in psychiatric disorders, Alzheimer's disease, and amyotrophic lateral sclerosis

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Subsequently, the cultures were incubated with antibody aggregates diluted 1:20 in Tyrode (untreated cultures were incubated in plain Tyrode)
