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The beads were incubated with the size standard DNAs (Table S2) containing an Atto555 dye on one end and cholesterol on the other in buffer containing 150 mM HEPES and 300mM NaCl for 10 minutes before imaging

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Culture and treatmenthuman differentiated neurons NBM complete media was prepared by adding 20% dextrose, GlutaMAX (Gibco 35050), and MEM NEAA (Invitrogen 11140-050) to pure NBM (Gibco 21103) to yield final concentrations of 0.3%, 2 mM, and 1, respectively

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