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Tissue sections were incubated with 3% H 2 O 2 in methanol for 20 min to block endogenous peroxidases

SDS-PAGE resolves this by denaturing all proteins into rod-shaped SDS-protein complexes with uniform charge density (~1 SDS per 2 amino acids), so that mobility in the polyacrylamide gel is determined solely by the sieving effect of the pore matrix, giving a linear relationship between log molecular weight and relative migration distance (Rf)

Sohail M, Pirzada T, Guenther R, Barbieri E, Sit T, Menegatti S, Crook N, Opperman CH, Khan SA

D., Alvarez, J

Similarly, the dependency of active sites of various fungal enzymes on the cysteinyl residues was confirmed for various fungal enzymes [17, 18, 22, 25, 27, 76]

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