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doi: 10.1172/JCI133270 14 LindqvistAAbelsMShcherbinaLNgaraMKryvokhyzhaDChriettSet al

Below are possible reasons why you may be getting low transfection efficiency, along with suggested solutions: Possible Cause: Plasmid DNA, siRNA, or transfection reagent diluted in media containing serum or complexes formed in the presence of serum Suggested Solution: Use serum-free medium for dilutions of plasmid DNA, siRNA, and transfection reagents

Impact of opioid therapy on gonadal hormones: Focus on buprenorphine

These infectious agents can upset the balance between health and disease

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Standard protocols using 1 to 2 mg daily are operating at a tiny fraction of these thresholds
