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70% ethanol, for example, will precipitate out the salt in 1.5 M NaCl, and that precipitation will clog your beads

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Isolated tryptic peptides were desalted by C18 SPE (Empore, 3M), dried under vacuum, and resuspended in 5% formic acid and 5% acetonitrile for analysis by LC/MS-MS on an Orbitrap Fusion mass spectrometer (Thermo Fisher Scientific) coupled to a Proxeon EASY-nLC II liquid chromatography (LC) pump (Thermo Fisher Scientific)

Solomon et al., 2014)

Glutathione-mediated response to acid stress in the probiotic bacterium, Lactobacillus salivarius