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Briefly, we separated proteins (60-100 g) in 10-12% SDS-PAGE in TRIS-Glycine-SDS buffer (Fisher Scientific, Hampton, NH, USA) to 100-120 V

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LD accumulation is consistently observed in hypoxic cancer cells, and hypoxia-related LD remodeling has also been described in colorectal cancer and salivary adenoid cystic carcinoma microenvironments, highlighting a conserved adaptive strategy in certain cancer types 92,93,94,95

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