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Rg 1 specifically upregulates activating transcription factor (ATF) 3/acyl-coenzyme A oxidase (ACOX) 2 to alleviate NAFLD, and how Rg 1 regulates NAFLD through these two genes is unknown ( 4.2.2 Oxidative stress The occurrence of OS in the body indicates that the antioxidant system scavenges ROS at a slower rate than the rate of ROS production, which is an important factor in liver injury and NAFLD progression (Kumar et al., 2021)

We amplified the cDNA of unc1 to clone it into pDONR 221P5P2 unc1 (pAPG2)

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