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Cytokine Growth Factor Rev (2017) 35:714.10.1016/j.cytogfr.2017.04.003 31 BingerKJCrte-RealBFKleinewietfeldM
Hormonal therapy is rarely given as a single agent and although can be effective, is often used to prevent or delay recurrence of cancer after other treatments have removed the majority of the cancer cells
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When confluency reached 70%, the media was changed by DMEM supplemented with 50 M biotin (Sigma-Aldrich) and incubated typically for 24 h
