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10.1016/j.biocel.2006.07.001 159
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Blots were incubated with HRP-conjugated secondary antibodies (BS13278, Bioworld Technology Inc., MN, USA) for 1 h, and protein expression was detected with ECL (WBULS0500, Millipore, MA, USA) by digital imaging systems (SYNGENE, Cambridge, UK)
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An obvious decrease in the fluorescent signal corresponding to NC correlated with decreasing amounts of oxidized lysozyme, indicating that NC-based assays can be developed for quantifying the amount of carbonyls on a protein
