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An integrated network pharmacology approach reveals that Darutigenol reduces inflammation and cartilage degradation in a mouse collagen-induced arthritis model by inhibiting the JAK-STAT3 pathway

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(New York, USA: Academic Press), 447503

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Cytoskeletal organization was assessed by F-actin staining using phalloidin-iFluor 532 (Cayman Chemical

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Twenty microliters from each fraction were analyzed by SDS-PAGE followed by silver staining, and densitometry was used to quantify the amount of -SNAP in each fraction

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The disease-linked variants exhibit reduced expression levels and decreased protein stability in the cell, but enzyme activity is preserved

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