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Membranes were blocked at room temperature for 2 h in a blocking buffer containing 1% bovine serum albumin to prevent nonspecific binding and then incubated with an appropriate primary antibody at 4C overnight (26, 43)

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These effects are particularly noticeable in individuals with a B12 deficiency or conditions like chronic fatigue syndrome or anemia, where long-term treatment can significantly improve quality of life

The H&E staining results (Fig

This is why we advocate for a proactive approach