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Rapid disruption of intestinal barrier function by gliadin involves altered expression of apical junctional proteins

Unlike the highly specific targeting of infectious agents by adaptive immune responses, ROS and RNS exhibit indiscriminate toxicity toward biological systems through their capacity to react with lipid, amino acid, and nucleic acid moieties that are conserved among both eukaryotic host and invading microbe (Patel et al

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The sample was vortexed for 30 s and incubated on ice for 30 min followed by incubation at -20 for 2 h

doi: 10.1016/j.cellimm.2022.104546 37 LagranhaCJLevada-PiresACSellittiDFProcopioJCuriRPithon-CuriTC

Free Radic Biol Med 27:922935 Griffith OW, Mulcahy RT (1999) The enzymes of glutathione synthesis: -glutamylcysteine synthetase