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This varies, but most protocols recommend a minimum of 8 to 12 weekly sessions as an initial course, followed by monthly maintenance

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The spectrophotometer (Shimadzu, model UV 1800, Kyoto, Japan) was used to record the catalase activity at an absorbance of 240 nm for 2 min ( 2.4.5 Determination of flavonoids contents The TFC contents were measured following the protocol of (Nongalleima et al., 2017)