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The highest dark and phototoxicity and ROS production were found for 7 (IC 50 = 23.17 and 6.28 M), and the efficiencies of 6 and especially 7 were sometimes lower

For soluble proteins, sandwich assay designscapture on the nanofiber surface plus a second detection binderprovide orthogonal recognition that reduces matrix-driven false signals while supporting electrochemical/optical readout