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Biological replicates (individual fish) are stated in the figure legends, and include (non-exhaustive): plasma TMT proteomics ( n = 5 adult versus n = 5 aged-adult male fish)

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These siRNA oligos were transfected into cells at 50% confluence for 6 h using Lipofectamine 3000 Kit (L3000015, Thermo Fisher Scientific, USA), following the manufacturers instructions

In addition to the pivotal role of GPX4, recent studies have identified several GPX4-independent pathways that regulate lipid peroxidation and ferroptosis, providing multilayered protection against oxidative stress

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Boosts cellular energy production and strengthens antioxidant defense