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RPTEC were maintained in Renal Epithelial Cell Basal Medium (REBM, ATCC) supplemented with 0.5% FBS, 10 nM triiodothyronine, 10 ng ml 1 rh EGF, 100 ng ml 1 hydrocortisone hemisuccinate, 5 g ml 1 rh insulin, 1 M epinephrine, 5 g ml 1 transferrin and 2.4 mM L-alanyl-L-glutamine
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