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indicated that lower plasma testosterone levels in older men are linked to a higher risk of AD, suggesting that testosterone deficiency is a potential risk factor for cognitive impairment [189]

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To investigate whether the increased GSH level at 100 g/ml 5-FOA (Figure S2A) was stable during longer-term cultivation of the modified strain, cellular GSH content was monitored every 10 generations during sequential batch cultivation in flasks at 100 g/ml 5-FOA (after every ~ 10 generations, aliquots of overnight culture were transferred to fresh medium giving OD 0.01 and re-incubated)

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H 2 O 2 stock solution (30%) was diluted in water immediately before the experiment

The solution is water-based and flows easily through even a 32G needle