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A0216, 1:7000) for 1 h at room temperature, washed three times with washing buffer, incubated with the western blotting substrate (Beyotime), and imaged with an imaging system (Thermo Fisher Scientific, iBright FL1000)

FIGURE 3 Another notable finding revealed by PET and NMR (nuclear magnetic resonance) spectroscopy analysis, is that in the brains of patients affected by ASD were found decreased glucose utilization and reduced ATP levels, further confirming mitochondrial dysfunction (Palmieri and Persico, 2010)

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Omega-3 fatty acid supplementation at a dose of 4 g daily reduced blood pressure in patients with CKD in a double-blind trial (7)

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