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Some users accidentally underdosing through calculation errors or improper injection technique

Blood (2016) 128:4531

age-matched vehicle-treated WT control mice

Suspect a spinal cord injury when:- A good neurological assessment is important to identify the signs, but cervical spine precautions should be followed in appropriate cases with distracting injuries

This can cause methyl trapping, where the excess methylfolate is unable to be utilised in the methionine cycle (through the MTR enzyme) as usable B12 levels are too low

A 2022 dose-response RCT (Yi et al., Geroscience) at 300/600/900 mg/day raised NAD+ dose-dependently with no safety issues at 60 days