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The trypsin reaction was stopped using cold 10% fatty acid free BSA in DPBS to remove any exogenous fatty acids

This is why nerve damage should never be viewed as a simple local problem

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For instance, an 8-week double-blind controlled trial showed that when the subjects used a cream containing 2% L-carnitine daily and combined it with aerobic exercise three times a week, the average local fat thickness decreased by 12%-18%, and the proportion of fatty acid oxidation increased by approximately 15%-20%

Schumann, E

and at min-30: LCR (-13.6%) and PLA (9.54%)