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Fresh HEK293T cells were transduced with pL302-HA-SUMO2 virus to generate a stable polyclonal cell line

[DOI] [PubMed] [Google Scholar] 95.Mortensen S.A., Rosenfeldt F., Kumar A., Dolliner P., Filipiak K.J., Pella D., Alehagen U., Steurer G., Littarru G.P., Investigators Q.S.S

To a solution of GnHCl (1.72 g) in 0.1 M, pH 7.4 phosphate buffer (1.80 mL) were added TCEPHCl (90.2 mg), sodium ascorbate (63.0 mg) and MPAA (100 mg) and the pH of the mixture was adjusted to 5.51 by addition of 6 M NaOH

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The owners lived on a farm and possessed an old bottle of Tl 2 SO 4 containing rodenticide (Luxan Thalliumsulfaat oplossing 5 g/L, Luxan, Barneveld, The Netherlands)

These fuse with the cell so that the ingredients can enter the cell directly