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It was demonstrated in animal studies that unabsorbed quaternary amines such as choline, phosphatidylcholine, betaine or l-carnitine can be metabolized by gut microorganisms to produce the intermediate compound trimethylamine (TMA)

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Subsequently, the undiluted samples were analyzed on an HPLC system (1200 series, Agilent Technologies Deutschland GmbH, Bblingen, Germany) with an organic acid resin column (300 mm 8 mm) and the corresponding pre-column (40 mm 8 mm) (Chromatographie-Service GmbH, Langerwehe, Germany) heated to 60C

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Acosta FM, Jia U-TA, Stojkova K, Howland KK, Guda T, Pacelli S, Brey EM, Rathbone CR (2021) Diabetic conditions confer metabolic and structural modifications to tissue-engineered skeletal muscle