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Nitrogen is the limiting factor for biomass formation in the composition of the M9 medium, which is why NH 4 Cl was supplemented in this experiment to double the concentration to 2 g L 1 to reach a higher biomass in this cultivation

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Test solutions Transfer 4.0 g of Levocarnitine to a 100-mL volumetric flask, dissolve in and dilute with water to volume, and mix to obtain a stock solution