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Rats were given unrestricted access to water and a standard diet ad libitum

doi: 10.1152/jappl.2000.88.2.690

A6 cell health was determined by staining with 50 L sterile filtered 0.5 g/mL DAPI (Invitrogen) for 5 min, after which the staining solution was removed and replaced with 100 L solution A

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