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Russell, et al
Frozen samples of culture medium were thawed on ice, and 2 l of 200 mM TCEP (in 125 mM borate buffer, pH 9.0) was added to 200 l of medium to yield a final concentration of 2 mM TCEP for reducing disulfide bonds
Nucleic Acids Res 35(21):71887196
However, it has certain limitations and disadvantages
The extent of the complex formation was controlled by changing two major factors: the duration of the first reduction and the ratio of Au to MHA (Supplementary Figs
