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At each time-point, cells were harvested using a 0.25% (v/v) trypsinEDTA solution, washed twice with PBS and centrifuged (300 g, 5 min, 20 C)

10.1111/j.1553-2712.2000.tb01889.x Acad Emerg Med

1990;58(Suppl 1):8188

After 24 h of treatment, we stained the cell nuclei with DAPI and confirmed the extent of EV uptake

Hepatic, drug-metabolizing cytochrome P450s (CYPs) catalyse the oxidation of tamoxifen to several metabolites