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We typically only do an extraction if its very superficial

Importantly, one study (89) found that in murine 3T3-derived cells, lipid accumulation was not observed without application of the induction cocktail in vitro (this was not investigated in other studies)

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We calculated integrated Nile Red fluorescence (excluding the non-specific stained particles) or lipid 2,845 cm 1 BCARS intensity of all intestinal lipid particles shown in each 2p-FLIM or BCARS image, and then normalized the integrated intensity by dividing the tissue area

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