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PMID: 28002060

Johansson et al

The influence of reactive oxygen species in the immune system and pathogenesis of multiple sclerosis

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In brief, the retinal tissues and primary microglia were washed twice with cold PBS and incubated in 100 L lysis buffer (T-PER Tissue Protein Extraction Reagent + 2% protease inhibitor) per whole retina explants on ice