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To substantiate the role of GSH in ABCC1-mediated resistance, cells were preincubated with buthionine-sulfoximine (BSO) (1 M, 18 h) prior to DpC treatment

Lancet 349 : 769771 Watson LM, Chan AK, Berry LR, Li J, Sood SK, Dickhout JG, Xu L, Werstuck GH, Bajzar L, Klamut HJ and Austin RC (2003) Overexpression of the 78-kDa glucose-regulated protein/immunoglobulin-binding protein (GRP78/BiP) inhibits tissue factor procoagulant activity

For the amplification of GLUT-4 and Ppia , specific primers were used with the following real-time PCR steps: an initial denaturation step followed by 40 cycles of denaturation, annealing, and extension

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doi: 10.1155/2012/736837 [DOI] [PMC free article] [PubMed] [Google Scholar] 14.Georgiou-Siafis SK, Tsiftsoglou AS

Membranes were blocked for 1 h at room temperature in 3% BSA prepared in TBS-T (50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 0.05% Tween 20)