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Blood samples were centrifuged approximately 60 minutes after collection, and serum cortisol levels were analyzed in an accredited diagnostic laboratory on a Cobas e411 analyzer using a competitive electrochemiluminescence immunoassay (Roche Diagnostics, Rotkreuz, Switzerland) with a coefficient of variation of 3.6% at 793 nmol/L

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They pass on the signal from the activated GPCRs to the cell interior and lead to a cascade of interactions and enzyme activities that ultimately brings about a change in cell function

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