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General anesthesia was achieved through initial inhalation of 4% (w/v) isoflurane, followed by ketamine/xylazine administration (60 mg/kg and 12 mg/kg, respectively) via intraperitoneal route, with concurrent subcutaneous buprenorphine for perioperative analgesia

(i) Specific affected brain areas (04) (score 0 indicates no histopathologic change), cerebral (NTP-7, Level 3), cerebellar cortex (NTP-7, Level 6), hippocampus, thalamus, and hypothalamus (NTP-7, Level 3) were assessed as follows: small, patchy, complete or incomplete infarcts (10% of area affected) (score 1)

The cell pellets were washed twice with 1 ml of 1 PBS

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Reviews in Endocrine and Metabolic Disorders 19, 293300