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Nobile V, Schiano I, Peral A, Giardina S, Spart E, Caturla N

A lack of glycine impairs collagen formation, and the body may suffer from weakening bones, joints and other connective tissues

[DOI] [PMC free article] [PubMed] [Google Scholar] 35.Serman L., Dodig D

2018. Therapeutic potential of NAD-boosting molecules in vivo

doi: 10.48130/FMR-2023-0021 74

Crystallisation To obtain peptide-free DAP BII crystals 39 , the samples were crystallised using the hanging-drop method, in which 1 l of protein solution (10 mg/ml DAP BII in 80 mM Tris-HCl, pH 8.5) was mixed with the same volume of reservoir solution (18% (w/v) PEG8000, 20% (v/v) glycerol and 2 mM zinc chloride in 0.08 M CHES-NaOH, pH 9.5) and incubated at 293 K