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TPP data acquisition: Nano-LC-MS / MS Nano-LCMS / MS analyses were performed on an Orbitrap Exploris 480 mass spectrometer (Thermo Fisher Scientific) coupled to an EASY-nLC HPLC system (Thermo Fisher Scientific)

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Over a full 24-hour period, GHK-Cu copper peptides and supporting extracts are released slowly and steadily, providing consistent support rather than short-lived bursts
Subsequently, network pharmacology and molecular docking identified SIRT1 as a potential target of GHK-Cu
