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At the cellular level, in vitro assays using cell lines (e.g., HEK293) transfected to express specific receptors like GLP-1R or MC4R are essential for characterizing ligand binding affinities, potency (EC50), and downstream signaling events like cAMP accumulation
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Abstract Nail matrix nevus is a type of benign melanocytic proliferation that presents as longitudinal melanonychia (LM) [1]

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