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From Raman and chromatographic analysis results, the lipid-storage particles are non-polar lipid-dominant, protein-poor, and with triacylglycerol/phospholipid ratio (TAG/PL) 20, while the yolk particles contain more polar lipids, more protein components, and with TAG/PL 0.5 ( 3.3 FLIM color representation In order to better visualize the acquired 2p-FLIM data, we performed HSV-RGB transformation