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When the skin encounters inflammation like acne, it needs to be treated gently as your skin barrier is damaged and needs to be repaired

After 30 min at room temperature, the fixed cells were washed twice with PBS 1X and then stained using Oil Red O, diluted 6/10 in distilled water, for 20 min at room temperature

Bacteriostatic water is that same sterile water plus 0.9% benzyl alcohol, which makes it a multi-dose container that tolerates repeated draws for aliquoting

The reason is that many bacteria contributed to the AMR burden, and each accounted for a small share